17-hydroxycorticosteroids urine test17-OH corticosteroids; 17-OHCS
The 17-hydroxycorticosteroids (17-OHCS) test measures the level of 17-OHCS in the urine.
How the Test is Performed
A 24-hour urine sample is needed. You will need to collect your urine over 24 hours. Your health care provider will tell you how to do this. Follow instructions exactly.
Collect your urine over 24 hours
The urine 24-hour volume test measures the amount of urine produced in a day. The amount of creatinine, protein, and other chemicals released into t...
How to Prepare for the Test
The provider will instruct you, if necessary, to stop medicines that may interfere with the test. These may include:
- Birth control pills that contain estrogen
- Certain antibiotics
How the Test will Feel
The test involves only normal urination. There is no discomfort.
Why the Test is Performed
17-OHCS is a product formed when the liver and other body tissues break down the steroid hormone cortisol.
This test can help determine if the body is producing too much cortisol. The test may be used to diagnose Cushing syndrome. This is a disorder that occurs when the body has a constant high level of cortisol.
Cushing syndrome is a disorder that occurs when your body has a high level of the hormone cortisol.
The urine volume and urine creatinine are often done with 17-OHCS test at the same time. This helps the provider interpret the test.
This test is not done often now. The free cortisol urine test is a better screening test for Cushing disease.
Cortisol urine test
The cortisol urine test measures the level of cortisol in the urine. Cortisol is a glucocorticoid (steroid) hormone produced by the adrenal gland. C...
- Male: 3 to 9 mg/24 hours (8.3 to 25 µmol/24 hours)
- Female: 2 to 8 mg/24 hours (5.5 to 22 µmol/24 hours)
Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your provider about the meaning of your specific test results.
What Abnormal Results Mean
A higher than normal level of 17-OHCS may indicate:
- A type of Cushing syndrome caused by a tumor in the adrenal gland that produces cortisol
- Hydrocortisone therapy
- A hormonal cause of severe high blood pressure
- Severe physical or emotional stress
- Tumor in the pituitary gland or elsewhere in the body that releases a hormone called adrenocorticotropic hormone (ACTH)
A lower than normal level of 17-OHCS may indicate:
- Adrenal glands are not producing enough of their hormones
- Pituitary gland is not producing enough of its hormones
- Hereditary enzyme deficiency
- Previous surgery to remove the adrenal gland
Urinating more than 3 liters a day (polyuria) can make the result of the test high even though cortisol production is normal.
There are no risks with this test.
Chernecky CC, Berger BJ. 17-hydroxycorticosteroids (17-OHCS) - 24-hour urine. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures. 6th ed. St Louis, MO: Elsevier Saunders; 2013:659-660.
Juszczak A, Morris DG, Grossman AB, Nieman LK. Cushing's syndrome. In: Jameson JL, De Groot LJ, de Kretser DM, et al, eds. Endocrinology: Adult and Pediatric. 7th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 13.
Review Date: 5/6/2019
Reviewed By: Brent Wisse, MD, Associate Professor of Medicine, Division of Metabolism, Endocrinology & Nutrition, University of Washington School of Medicine, Seattle, WA. Also reviewed by David Zieve, MD, MHA, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.